Respan Dataset Explorer

翻译Demographic data and sample characteristics
As shown in Table 1, sample t1-t5 represent abdominal aortic aneurysm tissue from patient, while b1-b5 represent adjacent normal aortic tissue from healthy donor. The average dose of IgE used to treat AAA patients is 713.6g/L. The other more detailed clinical diagnosis information was illustrated in the Table 1.

Analysis of circRNA expression profiles
A total of 6373 circRNAs were identified, including 3492 known circRNAs and 2881 New circRNAs (Figure 1A). The correlation scatter plot showed that the circRNAs expression profile was close between the two group samples (Figure 1B). The volcano plot clearly showed the number of circRNAs differentially expressed between AAA and normal arteries (Figure 1C). A total of 325 circRNAs were differentially expressed, of which 216 were up-regulated and 109 were down-regulated (Figure 1D, and Table S1). 

The function of circRNAs
Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway analyses were used to analyze the major functions of differentially expressed circRNAs (Figure 2). As a result, the most enriched biological process (BP) terms were chemical synaptic transmission, histone mRNA catabolic process, axo-dendritic transport, etc (Figure 2A). For cellular component (CC), these circRNAs were enriched in nucleus and perinuclear region of cytoplasm (Figure 2B). The most enriched molecular function (MF) terms were GTP binding, chaprone binding, RNA polymerase Ⅱ core binding and pritein binding (Figure 2C). KEGG analysis found that circRNAs were enriched in HTLV-I infection and central carbon metabolism in cancer (Figure 2D).

Construction of circRNA-miRNA-mRNA ceRNA interaction network  
As shown in Figure 3, We showed 10 differentially expressed circRNAs, 14 miRNAs and 10 DEMs participated in ceRNA network construction. In addition, we found circZEB1/miR-6749-3p/KLF4 axis might be a potential research target for AAA through further analysis of interaction between circRNA-miRNA and miRNA-mRNA (Table S2).

Functional enrichment analysis
As for the signal pathway of these differentially expressed circRNAs involved, we used GENCLIP3 to achieve functional enrichment analysis. We uploaded the genes into the GENCLIP3 search box, and found that the significant pathway was enriched in the pathways in cancer (KEGG) (P-Value = 0.038589) (Table S3).

人口统计数据和样本特征
如表1所示，样本t1-t5代表来自患者的腹主动脉瘤组织，而b1-b5代表来自健康捐赠者的相邻正常主动脉组织。用于治疗AAA患者的平均IgE剂量为713.6g/L。其他更详细的临床诊断信息见表1。

circRNA表达谱分析
共鉴定出6373个circRNA，包括3492个已知circRNA和2881个新circRNA（图1A）。相关性散点图显示两组样本之间的circRNA表达谱十分接近（图1B）。火山图清晰显示了AAA和正常动脉之间差异表达的circRNA数量（图1C）。共有325个circRNA表达差异，其中216个上调，109个下调（图1D和表S1）。

circRNA的功能
基因本体（GO）和京都基因与基因组百科全书（KEGG）通路分析用于分析差异表达circRNA的主要功能（图2）。结果显示，最富集的生物学过程（BP）术语包括化学突触传递、组蛋白mRNA降解过程、轴突-树突运输等（图2A）。在细胞成分（CC）方面，这些circRNA富集在细胞核和细胞质近核区域（图2B）。最富含的分子功能（MF）术语是GTP结合、分子伴侣结合、RNA聚合酶Ⅱ核心结合和蛋白质结合（图2C）。KEGG分析发现，circRNA在HTLV-I感染和癌症中的中心碳代谢中富集（图2D）。

构建circRNA-miRNA-mRNA ceRNA交互网络
如图3所示，我们展示了10个差异表达的circRNA、14个miRNA和10个DEM参与ceRNA网络构建。此外，我们通过进一步分析circRNA-miRNA和miRNA-mRNA之间的相互作用，发现circZEB1/miR-6749-3p/KLF4轴可能是AAA的潜在研究靶点（表S2）。

功能富集分析
对于这些差异表达的circRNA所涉及的信号通路，我们使用GENCLIP3来进行功能富集分析。我们将基因上传到GENCLIP3搜索框中，并发现显著通路在癌症路径（KEGG）中富集（P值=0.038589）（表S3）。

Hai Xin conducted bioinformatic analysis and wrote the draft. The other authors participated the discussion of our research. The corresponding auther administered the process of research. 

海欣进行了生物信息学分析并撰写了草稿。其他作者参与了我们研究的讨论。通讯作者管理了研究的过程。

Table S2 The detailed information of the cirRNA-miRNA-mRNA interaction network.
Table S3 The detailed information of related signal pathways of final circRNAs. 

表S2 circRNA-miRNA-mRNA相互作用网络的详细信息。
表S3 最终circRNA相关信号通路的详细信息。

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翻译：在这篇稿件中，我们探讨了我们发现了10个潜在的circRNA靶标，circZEB1/miR-6749-3p/KLF4轴可能是AAA研究的首选方向

在这篇稿件中，我们探讨了我们发现了10个潜在的circRNA靶点，circZEB1/miR-6749-3p/KLF4轴可能成为腹主动脉瘤（AAA）研究的优选方向。

翻译为SCI文章英语语言：我们发现了10个潜在的circRNA靶标，circZEB1/miR-6749-3p/KLF4轴可能是AAA研究的首选方向

We identified 10 potential circRNA targets, and the circZEB1/miR-6749-3p/KLF4 axis may be a preferred research direction for abdominal aortic aneurysm (AAA) studies.

翻译MiR-150 regulates the proliferation and migration of vascular smooth muscle cells and abdominal aortic aneurysm

MiR-150调节血管平滑肌细胞的增殖和迁移以及腹主动脉瘤。

翻译Backgrounds/Aims: Many microRNAs (miRNAs) could be used as the specific targets for the diagnosis and treatment of abdominal aortic aneurysm (AAA). miR-150-5p is reported to regulate the smooth apoptosis and proliferation muscle cell (VSMC). However, the exact role of miR-150-5p in regulating the development and progression of AAA is still unknown. 
Methods: Human specimens were used to verify the different expression of miR-150-5p in normal and AAA tissues. The cell counting kit 8 was used to determine the cell viabilities of transfected cells with up-regulated or down-regulated miR-150-5p expression. Wound healing assay and transwell assay were used to evaluate the proliferation and migration of VSMCs. The hypothetical targets for miR-150-5p were predicted using the bioinformatics analysis and the downstream targets of miR-150-5p were further verified via the quantitative real-time polymerase chain reaction (qRT-PCR) and Western Blot in VSMCs. Finally, this interaction was finally confirmed in both human and animal specimens AAA tissues. 
Results: The expression of miR-150-5p was significantly up-regulated in both human and mouse AAA models when compared with the corresponding control group. Up-regulation of miR-150-5p could greatly induce the proliferation and migration of VSMCs, while down-regulation its expression exhibited the opposite results. Based on gene interaction, EPHB2 and EGR2 were identified as direct downstream targets of miRNA-150-5p. 
Conclusions: The research shows that miRNA-150-5p played a protective role in the progression of AAA by targeting EPHB2 and EGR2, which providing a theoretical basis for seeking nucleic acid drugs to treat AAA.

背景/目的：许多微型RNA（miRNAs）可以作为腹主动脉瘤（AAA）诊断和治疗的特异性靶标。据报道，miR-150-5p能够调节平滑肌细胞（VSMC）的凋亡和增殖。然而，miR-150-5p在调控AAA发展和进展中的确切作用仍然未知。

方法：使用人类样本来验证正常和AAA组织中miR-150-5p的差异表达。使用细胞计数试剂盒8来确定转染上调或下调miR-150-5p表达的细胞活性。创伤愈合实验和Transwell实验用于评估VSMCs的增殖和迁移。利用生物信息学分析预测miR-150-5p的假设靶点，并通过定量实时聚合酶链反应（qRT-PCR）和Western Blot在VSMCs中进一步验证miR-150-5p的下游靶标。最后，这一相互作用最终在人类和动物AAA组织样本中得到证实。

结果：与相应对照组相比，在人类和小鼠AAA模型中miR-150-5p的表达均显著上调。miR-150-5p的上调能够显著促进VSMCs的增殖和迁移，而下调其表达则表现出相反的结果。基于基因相互作用，EPHB2和EGR2被识别为miRNA-150-5p的直接下游靶标。

结论：这项研究表明，miRNA-150-5p通过靶向EPHB2和EGR2在AAA进展中发挥了保护作用，为寻求核酸药物治疗AAA提供了理论依据。

翻译Backgrounds/Aims: Many microRNAs (miRNAs) could be used as the specific targets for the diagnosis and treatment of abdominal aortic aneurysm (AAA). miR-150-5p is reported to regulate the smooth apoptosis and proliferation muscle cell (VSMC). However, the exact role of miR-150-5p in regulating the development and progression of AAA is still unknown. 
Methods: Human specimens were used to verify the different expression of miR-150-5p in normal and AAA tissues. The cell counting kit 8 was used to determine the cell viabilities of transfected cells with up-regulated or down-regulated miR-150-5p expression. Wound healing assay and transwell assay were used to evaluate the proliferation and migration of VSMCs. The hypothetical targets for miR-150-5p were predicted using the bioinformatics analysis and the downstream targets of miR-150-5p were further verified via the quantitative real-time polymerase chain reaction (qRT-PCR) and Western Blot in VSMCs. Finally, this interaction was finally confirmed in both human and animal specimens AAA tissues. 
Results: The expression of miR-150-5p was significantly up-regulated in both human and mouse AAA models when compared with the corresponding control group. Up-regulation of miR-150-5p could greatly induce the proliferation and migration of VSMCs, while down-regulation its expression exhibited the opposite results. Based on gene interaction, EPHB2 and EGR2 were identified as direct downstream targets of miRNA-150-5p. 
Conclusions: The research shows that miRNA-150-5p played a protective role in the progression of AAA by targeting EPHB2 and EGR2, which providing a theoretical basis for seeking nucleic acid drugs to treat AAA.

背景/目的：多种微型RNA（miRNAs）可被用作腹主动脉瘤（AAA）的诊断和治疗的特异性靶标。miR-150-5p已被报道调控平滑肌细胞（VSMC）的凋亡和增殖。然而，miR-150-5p在调节AAA的发展和进程中的确切作用仍不明确。

方法：使用人类样本验证正常和AAA组织中miR-150-5p的表达差异。采用细胞计数试剂盒8评估转染后表达上调或下调miR-150-5p的细胞活力。创伤愈合实验和Transwell实验用以评估VSMCs的增殖和迁移。使用生物信息学分析预测miR-150-5p的假定靶点，并通过定量实时聚合酶链反应（qRT-PCR）和Western Blot在VSMCs中进一步验证miR-150-5p的下游靶标。最终在人类和动物AAA组织样本中证实了这一相互作用。

结果：与相应的对照组相比，人类和小鼠AAA模型中miR-150-5p的表达显著上调。miR-150-5p的上调能显著诱导VSMCs的增殖和迁移，而其表达的下调则产生相反效果。基于基因互作，EPHB2和EGR2被鉴定为miR-150-5p的直接下游靶标。

结论：研究表明，miR-150-5p通过靶向EPHB2和EGR2，在AAA的发展过程中发挥了保护作用，为寻找治疗AAA的核酸药物提供了理论基础。